Search results for " in vitro culture."
showing 9 items of 9 documents
Seed mass variation and in vitro embryos culture of Abies nebrodensis (Lojac.) Mattei
2009
In vitro response of two Sicilian genotypes of Morus (L.) through axillary bud culture.
2007
A protocol for the in vitro establishment of two Sicilian genotypes of mulberry has been set up. Multiple shoots were initiated and plantlets were produced from buds collected from mature trees of Morus alba L. and Morus nigra L., grown in the field. The influence of two explanting times (September and December) of buds on the in vitro response has been observed. The percentage of sprouting after four weeks of culture was greatly higher with material explanted from the field in September compared with the one collected in December. Morus nigra L. produced more vigorous shoots than Morus alba L.. After the second subculture the number of shoots produced decreased, in both genotypes. No diffe…
Analisi delle popolazioni siciliane di Helichrysum: propagazione in vitro e indagini fitochimiche
2004
The Sicilian populations of Helichisum have got a germoplasm from which it is possible to select ornamental plants to be placed in a mediterranean and/or dry climate. The in vitro trials show an attitude for microprapagation and multiplication for commerciaI productions that can be used also for the re·introduction in the natural habitat. The highest multiplication rate belongs to three genotypes: H. hyblaeum (clone S6), H. nebrodense (Clone Cli) and H. rupestre var. rupestre (clone S2, S11). The height and the feature of the vitro plants are pecuIiar for each different clone. The clone S11 is suitable for the production of pot plants or bushes, the clone S2 and S6 for garden edge. The clon…
OCCURRENCE OF SPONTANEOUS TRIPLOIDS FROM SMALL SEEDS OF 'HERNANDINA' CLEMENTINE
2011
In vitro rooting and acclimatization of Sicilian Hypericum perforatum
2015
Hypericum perforatum L. is traditionally used as a medicinal plant because of different bioactive compounds with documented antidepressant and anti-inflammatory activities. Plantlets mass production with high content of these secondary metabolites has been enhanced through in vitro culture but often the process has been stopped at the multiplication phase. A study was conducted in order to set up an efficient in vitro rooting and acclimatization protocol of a H. perforatum Sicilian genotype well-adapted to south Mediterranean conditions. Aseptic nodal segments were cultured on to a Murashige and Skoog (MS) basal medium supplemented with 4.44 μM 6-benzyladenine (BA) for multiplication. Micro…
Ploidy manipulation for genetic improvement in some Mediterranean fruit crops
2017
Plant breeding is focused on selection of new genotypes with improved traits. Conventional methods based on hybridization and those based on biotechnology (somatic hybridization, genetic transformation, ploidy manipulation, etc.) are used to create novel genetic variations. Biotechnology provides powerful tools for plant breeding, for instance, haploid technology allows achievement of homozygous lines from heterozygous parents in one step, which reduces significantly the time required by conventional methods. Concerning woody species, characterized by self-incompatibility, long juvenile period and high degree of heterozygosity, this technique is the only way to get homozygous lines. Haploid…
Somatic embryogenesis and flow cytometric assessment of nuclear genetic stability for Sansevieria spp.: an approach for in vitro regeneration of orna…
2023
Sansevieria Thunb. species are traditionally known as succulent ornamental plants worldwide. They are also cultivated for medicinal, fodder, soil conservation and fiber uses, and for their capacity to reduce environmental pollution. Sansevieria sexual propagation is limited by the lack of viable seeds, and reproduction is largely made via vegetative propagation by suckers or cuttings. For these reasons, genetic improvement by conventional breeding is limited. To overcome this problem and to address the increasing demand from customers for novel Sansevieria varieties, many commercial companies regularly use in vitro propagation, as is the case in the breeding process of several ornamental pl…